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	Provedor de dados OAK (2) Autor INOUE, Noboru (2) SUGIMOTO, Chihiro (2) THEKISOE, Oriel M. M (2) 井上, 昇 (2) BAZIE, Raoul S. B (1) CORONEL-SERVIAN, Andrea M (1) JING, Zhang (1) KAWAZU, Shin-ichiro (1) MAGONA, Joseph W (1) OTIM, Charles P (1) SAKURAI, Tatsuya (1) Mais... Palavra-chave Bovine (1) DNA template (1) Diagnosis (1) LAMP (1) Parasitic disease (1) Prevalence (1) Trypanosoma (1) Trypanosome (1) Uganda (1) Zoonotic parasite (1) Mais... Ano 2009 (2) País Japan (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda OAK JING, Zhang; MAGONA, Joseph W; SAKURAI, Tatsuya; THEKISOE, Oriel M. M; OTIM, Charles P; SUGIMOTO, Chihiro; INOUE, Noboru; 井上, 昇. Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent... Palavras-chave: Bovine; Prevalence; Trypanosome; Uganda; Zoonotic parasite. Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2690 Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates OAK THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇. This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored... Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma. Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691 Registros recuperados: 2 Primeira ... 1 ... Última

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